A standardized whole cranberry extract (WCE) was used to stabilize a model sunflower-casein emulsion prototype for future formulation activities with a fresh cream cheese product. The WCE contained total organic acids (20% w/w) and polyphenols (5%), the latter consisting of total anthocyanins (10%, w/w) and proanthocyanidins (12% w/w). Antioxidant capacity of the WCE was determined by ORAC, (hydrophilic ORAC = 348.31 ± 33.45 μmol of Trolox equivalents/g; lipophilic ORAC = 11.02 ± 0.85 μmol of Trolox equivalents/g). WCE was effective at stabilizing the model emulsion at a level of 0.375% (w/w), yielding a final pH of 5.6. Generation of initial lipid peroxidation products, hexanal and pentanal was inhibited by 92.4% ± 3.9% and 66.6% ± 5.3% ( n = 3), respectively, when emulsions containing WCE were incubated at 50 °C for 90 h. This information was useful for formulating a fresh cream cheese product containing WCE to produce value-added potential and good self-life. The standardized WCE gave a final pH of 5.6 for the cheese premix and also significantly ( P < 0.05) lowered both the PV and CD after 28 and 21 days at 4 °C storage, respectively, compared to untreated control. We conclude that there are important functional role(s) for cranberry constituents when presented as a standardized ingredient for producing value-added, stable fresh dairy products.
Moore, R.L.; Duncan, S.E.; Rasor, A.S.; Eigel, W.N.; O’Keefe, S.F. Oxidative stability of an extended shelf-life dairy-based beverage system designed to contribute to heart health. J. Dairy Sci. 2012, 95, 6242–6251, doi:10.3168/jds.2012-5364.
McClements, D.J.; Decker, E.A. Lipid oxidation in oil-in-water emulsions: Impact of molecular environment on chemical reactions in heterogeneous food systems. J. Food Sci. 2000, 65, 1270–1282, doi:10.1111/j.1365-2621.2000.tb10596.x.
Soto-Cantu, C.D.; Graciano-Verdugo, A.Z.; Peralta, E.; Islas-Rubio, A.R.; Gonzalez-Cordova, A.; Gonzalez-Leon, A. Release of butylated hydroxytoluene from an active film packaging to asadero cheese and its effect on oxidation and odor stability. J. Dairy Sci. 2008, 91, 11–19, doi:10.3168/jds.2007-0464.
Vvedenskaya, I.O.; Vorsa, N. Flavonoid composition over fruit development and maturation in american cranberry, vaccinium macrocarpon ait. Plant Sci. 2004, 167, 1043–1054, doi:10.1016/j.plantsci.2004.06.001.
Satue-Gracia, M.T.; Heinonen, M.; Frankel, E.N. Anthocyanins as antioxidants on human low-density lipoprotein and lecithin-liposome systems. J. Agri. Food Chem. 1997, 45, 3362–3367, doi:10.1021/jf970234a.
Seeram, N.P.; Nair, M.G. Inhibition of lipid peroxidation and structure-activity-related studies of the dietary constituents anthocyanins, anthocyanidins, and catechins. J. Agri. Food Chem. 2002, 50, 5308–5312, doi:10.1021/jf025671q.
？kerget, M.; Kotnik, P.; Hadolin, M.; Hra？, A.R.; Simoni？, M.; Knez, ？. Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chem. 2005, 89, 191–198, doi:10.1016/j.foodchem.2004.02.025.
Xagorari, A.; Papapetropoulos, A.; Mauromatis, A.; Economou, M.; Fotsis, T.; Roussos, C. Luteolin inhibits an endotoxin-stimulated phosphorylation cascade and proinflammatory cytokine production in macrophages. J. Pharmacol. Exp. Ther. 2001, 296, 181–187.
Youdim, K.A.; McDonald, J.; Kalt, W.; Joseph, J.A. Potential role of dietary flavonoids in reducing microvascular endothelium vulnerability to oxidative and inflammatory insults. J. Nutr. Biochem. 2002, 13, 282–288, doi:10.1016/S0955-2863(01)00221-2.
Seeram, N.P.; Adams, L.S.; Hardy, M.L.; Heber, D. Total cranberry extract versus its phytochemical constituents: Antiproliferative and synergistic effects against human tumor cell lines. J. Agric. Food Chem. 2004, 52, 2512–2517, doi:10.1021/jf0352778.
Lee, C.H.; Reed, J.D.; Richard, M.P. Ability of various polyphenolic classes from cranberry to inhibit lipid oxidation in mechanically separated turkey and cooked ground pork. J. Muscle Foods 2006, 17, 248–266, doi:10.1111/j.1745-4573.2006.00048.x.
Kristensen, D.; Hansen, E.; Arndal, A.; Trinderup, R.A.; Skibsted, L.H. Influence of light and temperature on the color and oxidative stability of processed cheese. Int. Dairy J. 2001, 11, 837–843, doi:10.1016/S0958-6946(01)00105-4.
Van Aardt, M.; Duncan, S.E.; Marcy, J.E.; Long, T.E.; O'Keefe, S.F.; Nielsen-Sims, S.R. Aroma analysis of light-exposed milk stored with and without natural and synthetic antioxidants. J. Dairy Sci. 2005, 88, 881–890, doi:10.3168/jds.S0022-0302(05)72754-5.
Singleton, V.L.; Orthofer, R.; Lamuela-Raventos, R.M. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Method. Enzymoly 1999, 299, 152–177, doi:10.1016/S0076-6879(99)99017-1.
Wrolstad, R.E.; Giusti, M.M. Characterization and measurement of anthocyanins by UV-visible spectroscopy. In Food Analytical Chemistry; Wrolstad, R.E., Ed.; John Wiley & Sons, Inc.: New York, NY, USA, 2001.
Gessner, M.O.; Steiner, D. Acid butanol assay for proanthocyanidins (condensed tannins). In Methods to Study Litter Decomposition: A Practical Guide; Graca, M.A.S., Barlocher, F., Gessner, M.O., Eds.; Springer Verlag GmbH: Heidelberg, Germany, 2005; pp. 107–114.
Bot, A.; Kleinherenbrink, F.A.M.; Mellema, M.; Magnani, C.K. Cream cheese as an acidified protein-stabilized emulsion gel. In Handbook of Food Products Manufacturing; Hui, Y.H., Ed.; Wiley-Interscience: New York, NY, USA, 2007; pp. 651–672.