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Sporulation and mycelial growth of Fusarium solani in different culture media and steady bright = Esporula o e crescimento micelial de Fusarium solani em diferentes meios de cultura e regimes de luminosidade

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Abstract:

Considering the physiological characterization of Fusarium solani isolated from cassava roots, the objective of this study was to evaluate mycelial growth and sporulation of F. solani in different culture media and lighting regimes. The fungus was grown using five culture media (potato dextrose agar, potato sucrose agar, cassava, agar-agar, and water micophil) under three light regimes (continuous darkness, a photoperiod of 12 h, and continuous light) during the incubation period of seven day, temperature 25 °C ± 2 °C. The trial was done in completely randomized design with three replications. Discs of 5 mm diameter taken from the edge of the colony grown on PDA medium were transferred to the center of Petri dishes containing 20 mL of each medium. Mycelial growth was determined by measuring the diameter of the colonies in two diametrically opposite directions while sporulation by quantifying conidia by drop method. No significant changes in the production of conidia and mycelial mass in different culture media and lighting regimes tested, and BDA and BSA under the regime of continuous light best sporulation and conidial production were observed. While in the midst AA under continuous darkness was the lowest rates of mycelial growth and sporulation. = Visando a caracteriza o fisiológica de Fusarium solani isolado de raízes de mandioca, objetivou-se com este trabalho avaliar a esporula o e o crescimento micelial de F. solani em diferentes meios de cultura e regimes de luminosidade. O fungo foi cultivado utilizando cinco meios de cultura (batata dextrose ágar, batata sacarose ágar, mandioca ágar, micophil e ágar-água) sob três regimes de luminosidade (escuro contínuo, fotoperíodo de 12 h e luz contínua) durante o período de incuba o de sete dias, a temperatura de 25 °C ± 2 o C. O ensaio foi conduzido em delineamento inteiramente casualizado, em esquema fatorial (5x3), com três repeti es. Discos de 5 mm de diametro, retirados da borda da col nia cultivada em meio BDA, foram transferidos para o centro de placas de Petri contendo 20 mL de cada meio. Determinou-se o crescimento micelial por meio da medida do diametro das col nias em dois sentidos diametralmente opostos, enquanto a esporula o por meio da quantifica o de conídios pelo método da gota. Foram observadas varia es significativas na produ o de massa micelial e conídios nos diferentes meios de cultura e regimes de luminosidade testados, sendo que BDA e BSA sob regime de luz contínua induziram maior crescimento micelial e produ o de conídios. Enquanto que no meio AA sob escuro contínuo ocorreu

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