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Separating climate resilient crops through screening of drought tolerant rice land races in Nepal
RR Puri, K Khadka, A Paudyal
Agronomy Journal of Nepal , 2010, DOI: 10.3126/ajn.v1i0.7546
Abstract: Many studies have shown that local landraces are found to be better adapted to changing climatic conditions. The screening of local land races is one of important processes to detect drought tolerant behavior of crop species to further verify in similar field situation and genomic/molecular level studies. In 2009 for main season rice, a study was done to identify different land races of rice in Jhapa and Kailali districts to know their performance on drought stressed conditions by field techniques. Among the 22 rice land races studied, Kataush showed maximum drought tolerant efficiency (DTE) of 192.8% and minimum drought susceptibility index (DSI) of -0.88% followed by Guthanisaro (DTE=181% and DSI= -0.77%). Likewise, in the cluster analysis, these genotypes formed a cluster with two highest DTE and minimum DSI. Hence, these two land races could be used for drought stressed breeding program in the future to include drought resistant genes for variety impovement. Also, it was recorded that these land races could be used as the most drought tolerant variety in Terai region similar to Jhapa and Kailali, Nepal. These varieties could play important role in climate extreme situations, where farmers can adopt them as climatic resilient rice genotypes. DOI: http://dx.doi.org/10.3126/ajn.v1i0.7546 Agronomy Journal of Nepal (Agron JN) Vol. 1: 2010 pp.80-84
Angiographic Evaluation of Coronary Collateral Circulation Patterns in Patients with Coronary Artery Disease Requiring Surgical Revascularization  [PDF]
Deepak Puri, Nidhi Puri
World Journal of Cardiovascular Diseases (WJCD) , 2017, DOI: 10.4236/wjcd.2017.711036
Abstract: Background: Coronary artery disease is highly prevalent in India with onset at a younger age. Coronary collateral circulation plays an important role in protecting myocardium from infarction, preserving myocardial contractility and reducing cardiovascular events. The objective of the present study is to assess the pattern of coronary collateral circulation in known cases of coronary artery disease with correlation to age, gender and degree of occlusion. Method: This is a retrospective study done on 200 preoperative angiograms in patients with coronary artery disease. Patients were classified according to age, gender and degree of obstruction in major vessels. Collateral vessels were graded according to the Rentrop classification. Patients with collaterals were further classified on the basis of intensity of collaterals into 3 groups: those with no collateralization (Grade 0), poor collateralization (Grade 1) and those with adequate collateralization (Grade 2 - 3). Results: Collateral development was seen in 175 (87.5%) angiograms. Collaterals were seen in 66% for left anterior descending (LAD), 44.5% for circumflex (LCx) and 70.5% for right coronary artery (RCA) block. Coronary collaterals between LAD and posterior descending artery (PDA) via ventricular septal branches were most common pathways. Adequate collaterals were seen in 53% males and 29.3% females (p < 0.001), 53.14% in patients of age > 50 years and in 32% in cases with age < 50 years (p = 0.014) and 54.8% cases with arterial obstruction > 90%. Conclusion: Collateral circulation between LAD and PDA via ventricular septal branches is the commonest pathway to develop. Although prevalence of LAD occlusion is higher but collaterals develop more for RCA occlusion. Adequate collaterals develop more frequently in males, cases above 50 years and in vessels with >90% obstruction.
Morpho-physiology of wheat genotypes under different sowing dates as affected by Helminthosporium leaf blight and leaf rust in Chiwan, Nepal
SP Neupane, BR Ojha, SK Ghimire, SK Sah, SM Shrestha, RR Puri
Agronomy Journal of Nepal , 2013, DOI: 10.3126/ajn.v3i0.9012
Abstract: An experiment was conducted at the Agronomy farm of the Institute of Agriculture and Animal Science (IAAS), Rampur, Nepal in 2008/09. The experiment was laid out in factorial design in spit plot arrangement consisting of date of sowing as main factor and wheat genotypes as sub factor. Wheat genotypes were sown in 22 November for normal sowing and 29 December for late sowing condition. These were assessed against Helminthosporium leaf blight and leaf rust severity in a naturally inoculated environment. Result showed that wheat genotypes differed significantly with respect to flag leaf area, flag leaf duration and plant height. Genotype BL-3064 showed the lower decline (624.9 to 615.1) in chlorophyll content whereas RR-21 showed highest decline (471.2 to 360.4) in chlorophyll content from normal to late sowing, possibly contributing to resistant to the disease. The AUDPC value revealed that genotypes BL-3625 and BL-3623 had increased level of resistance due to lower AUDPC value and higher flag leaf duration. The results of this study suggest that genotypes BL-3623, BL-3625, BL-3063 and BL-3064 could be the option in warm and humid regions of Terai condition so far as resistant to Helminthosporium leaf blight and leaf rust are concerned.
Gankyrin: A potential target for drug therapy against hepatocellular carcinoma  [PDF]
Shashank Gandhi, Veena Puri, Sanjeev Puri
Journal of Biomedical Science and Engineering (JBiSE) , 2012, DOI: 10.4236/jbise.2012.58059
Abstract: Hepatocellular Carcinoma is a primary malignant tumor of the liver and gankyrin is an oncoprotein over-expressed in hepatocellular carcinoma. It has been found that Gankyrin protein reduces the level of p53 protein by increasing its ubiquitylation and degradation, following a MDM-2 mediated pathway. Interaction of gankyrin with MDM2 enhances the ubiquitylation of p53. Independent study of this protein molecule revealed that it is identical to the p28 subunit of the 26S proteasome, having seven similar alpha helical ankyrin repeats. Gankyrin also binds to the Tumor Suppressor Protein (TSP) Retinoblastoma (RB), thereby accelerating its phosphorylation and proteasomal degradation. Blocking the expression of Gankyrin with MDM2 in cases of Hepatocellular Carcinoma (HCC) promoted apoptosis in cancer cells. Hence, Gankyrin can be used as a potential target for drug therapy against Hepatocellular Carcinoma.
A study on non venereal genital dermatoses in north India
Neerja Puri,Asha Puri
Our Dermatology Online , 2012,
Abstract: Non-venereal dermatoses of female external genitalia include a spectrum of diseases with varied etiology. The most common non-venereal dermatoses in males were scrotal dermatitis seen in 16.6% patients, vitiligo was seen in 14.3 % patients, fixed drug eruption ,scabies and pearly penile papules were seen in 10% patients each. Sebaceous cyst, tinea, psoriasis and lichen planus was seen in 6.6% patients each. Balanitis xerosus obliterans, squamous cell carcinoma and verrucous carcinoma and achrochordon were seen in 3.3% patients each. The most common genital dermatoses seen in females were lichen sclerosus (15%), vitiligo (15%) and vulval candidiasis in 15% cases. Other non venereal genital dermatoses in females were lichen simplex atrophicus (10%), bartholin cyst (10%), tinea (10%), psoriasis (10%), vulval lymphoedema (10%) and achrochordon in 5% patients.
A study on tinea capitis in the pre school and school going children
Neerja Puri,Asha Puri
Our Dermatology Online , 2013,
Abstract: Introduction: Tinea capitis is a superficial fungal infection of scalp and hair caused by various species of dermatophytes. The incidence of Tinea capitis varies from country to country and region to region.Material and Methods: tract Fifty patients from the preschool going population were selected for the study.Results and Discussion: Clinical presentation of disease revealed that black dot to be the commonest (32%) followed by grey patch (28%), kerion (20%) and favus type was the least (1%). Direct microscopy of hair in KOH preparations revealed that all clinically suspected patients of Tinea capitis had endothrix type in 56% of cases and ectothrix type in 44%.
A comparative study on 100% tca versus 88% phenol for the treatment of vitiligo
Neerja Puri,Asha Puri
Our Dermatology Online , 2012,
Abstract: There are various medical and surgical modalities for the treatment of vitiligo. Surgical modalities are used in the patients who fail to respond to medical therapy. We selected thirty patients of stable vitiligo from the department of dermatology for the study. The patients were divided into two groups of 15 patients each. In Group I patients application of 100% TCA was done on the vitiliginous sites and in Group II patients 88% phenol was applied on the affected sites. Comparing the results of repigmentation in both the groups it was seen that marked pigmentation was seen in 66.6% patients in the TCA group and 80% in the Phenol group. Moderate pigmentation was seen in 13.3% patients in both the groups and mild pigmentation was seen in 20% patients in the TCA group and 6.6% in the Phenol group.
A study on efficacy of oral zinc therapy for treatment of acrodermatitis enteropathica
Neerja Puri,Asha Puri
Our Dermatology Online , 2013,
Abstract: Introduction: Acrodermatitis enteropathica (AE) is a disorder of zinc metabolism that occurs in one of two forms: an inborn (congenital) form and an acquired form. The inborn form of AE is a rare genetic disorder characterized by intestinal abnormalities that leads to inability to absorb zinc from the intestine. The lack of zinc presents, characteristically, as skin inflammation with pustules occurring around the mouth and/or anus, diarrhea.Aims: To study the efficacy of oral zinc therapy on thirty patients of acrodermatitis enteropathica.Methods: Thirty clinically diagnosed patients of acrodermatitis enteropathica were taken for the study. The criterion of diagnosis of this condition was the clinical picture of symmetrical vesiculo-pustular dermatitis, in upper and lower limbs and periorificial regions.Results and Discussion: In our study maximum (50%) patients were below 1 year of age, 33.3% patients were between 13-24 months of age, 6.66% patients were between 25- 36 months and 37- 48 months each and 3.33% patients were between 49- 60 months of age. Nail changes were seen in 60% children. Also, it was seen that perioral area was the commonest (86.6%) site involved, followed by anogenital area in 80% patients, palms and soles were involved in 66.6% patients, arms in 46.6% patients and legs were seen in 40% patients. Regarding clinical features, dermatitis was seen in 100%patients, alopecia was seen in 40% patients, diarrhea in 60% patients and mental disturbances were seen in 30 % patients.
A study on dermatoses of pregnancy
Neerja Puri,Asha Puri
Our Dermatology Online , 2013,
Abstract: Certain dermatoses are specifically seen in pregnancy or postpartum period. It is therefore important for the clinicians to recognize and treat these cutaneous disorders to minimize maternal and fetal morbidity. The commonest pregnancy related dermatoses was polymorphic eruption of pregnancy seen in 22% patients, prurigo of pregnancy was seen in 7% patients, pemphigoid gestationis was seen in 3% patients, pruritic folliculitis of pregnancy was seen in 2% patients and intrahepatic cholestasis was seen in 1% of patients. It was seen that the skin disorders were commonest in the third trimester (60%), followed by 31% patients in second trimester and 9% patients in first trimester.
Strict anatomical co existence and colocalization of vitiligo and psoriasis – a rare entity
Neerja Puri,Asha Puri
Our Dermatology Online , 2013,
Abstract: The coexistence of psoriasis and vitiligo is rare. We describe a case report of a 58 year old female patient who developed typical psoraiatic plaques covering completely or partly the vitiliginous areas of her skin. Her psoriasis was strictly limited to the vitiliginous patches with no involvement of the normal skin. Strict anatomical coexistence of both diseases is extremely rare and suggests a casual mechanism, possibly due to a koebner phenomenon but genetic and environmental factors may also be involved.
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