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Callus formation in vitro and internodal stem apices in savory = Calogênese in vitro de segmentos apicais caulinares e internodais em segurelha (Satureja hortensis L.)
Marcio Carlos Navroski,Daniel Arthur Gaklik Waldow,Mariane de Oliveira Pereira,Alessandra de Oliveira Pereira5
Agro@mbiente On-line , 2012,
Abstract: We sought to evaluate with this work different growth regulators on callus formation in shoot apical and internodal stem segments of Satureja hortensis. The explants were isolated from in vitro seedlings and cultured on MS nutrient medium supplemented with NAA (0 and 1 μM) and BAP (0, 5, 10, 15 and 20 μM). The presence of auxin NAA gave higher marks to the calluses at 30 days of evaluation, these notes also increased with the addition of BAP. There were significant interactions between factors in evaluating NAA and BAP for 60 days, both in apical stem segments as in internodal stem segments. To stemapices percentage of friable callus tends to decrease with increasing concentration of BAP. As for compact calluses increased BAP leads to an increase in the percentage of this type of callus. The presence of NAA increased callus formation in both friable and compact calluses on. This trend was also observed in internodal stem segments. The callus formation was highly rhizogenic observed in the presence of NAA, hardly occurs in the absence of auxin. The use of BAP is recommended in case of regeneration of plants through micropropagation, if the goal is the production of metabolites, the use of BAP can be harmful by reducing the production of friable callus.= Buscou-se com este trabalho avaliar diferentes reguladores de crescimento sobre a calogênese em segmentos caulinares apicais e internodais de Satureja hortensis L.. Os explantes foram isolados de plantulas germinadas in vitro e cultivados em meio nutritivo MS acrescido de ANA (0 e 1 μM) e de BAP (0; 5; 10; 15 e 20 μM). A presen a da auxina ANA proporcionou notas mais altas para os calos aos 30 dias de avalia o, estas notas também aumentaram com o acréscimo da citocinina BAP. Houve intera es significativas entre os fatores ANA e BAP na avalia o aos 60 dias, nos dois tipos de segmentos caulinares(apicais e internodais). Nos segmentos apicais caulinares a porcentagem de calos friáveis tendeu a diminuir com o aumento da concentra o de BAP. Já nos calos compactos o aumento de BAP ocasionou um aumento da porcentagem deste tipo de calo. A presen a de ANA elevou a forma o tanto em calos friáveis como em calos compactos. Esta tendência também foi observada em segmentos caulinares internodais. A forma o de calos rizogênicos foi altamente observada na presen a de ANA, ase n o ocorrendo na ausência desta auxina. A utiliza o de BAP é recomendada em caso de regenera o de plantas por micropropaga o, no entanto, se o objetivo for à produ o de metabólitos, a utiliza o de BAP pode ser prejudicial por diminuir a
Assessment of Acute Episodes in Chronic Stable Angina: A Clinical-Laboratory Approach to the Use of the Coronary Vasodilator Propatyl Nitrate  [PDF]
Gerson Goldwasser, Renato Kaufman, Carlos Pereira Nunes, Alexandre Hid, Stephanie Wrobel Goldberg, Alessandra Santos, Lisa Oliveira, Adenilson de Souza da Fonseca, Mauro Geller
World Journal of Cardiovascular Diseases (WJCD) , 2016, DOI: 10.4236/wjcd.2016.67027
Abstract: Background/Objectives: Propatyl nitrate is a coronary vasodilator with immediate and prolonged action, indicated in the treatment and prevention of acute angina pectoris episodes. Methods: This was an open, self-paired comparative study performed at UNIFESO Medical School evaluating the clinical and laboratory results of treatment with propatyl nitrate in patients with chronic stable angina pectoris. Subjects received 10 mg of propatyl nitrate, at the dose of three sublingual tablets per day, to be taken at 8:00 A.M., 2:00 P.M., and 8:00 P.M. Subjects returned to the study center after 15 days of treatment for Visit 2 assessments, and at the end of the 30-day treatment period (Visit 3). Results: A total of 200 subjects were included in the study. There was a statistically significant reduction in blood pressure (p < 0.0001) and heart rate (p = 0.0001), but no change in respiratory rate (p = 0.23). Laboratory results did not vary throughout the treatment period. There was no significant change from pretreatment in the SAQ Physical Limitation scale (p = 0.7415). The Angina Stability, Angina Frequency, and Treatment Satisfaction, and Quality of Life scales showed a significant improvement from pretreatment (p < 0.0001). Adverse events were observed among 41 subjects at Visit 2 and 35 subjects at Visit 3. Conclusions: Propatyl nitrate was safe and effective in treating chronic stable angina pectoris over the course of the 30-day treatment period. Treatment with propatyl nitrate increased angina stability and reduced angina frequency while increasing treatment satisfaction and quality of life in the patient population evaluated.
The use of alcohol by workers of the garbage collection service
Mabuchi, Alessandra dos Santos;Oliveira, Daniel Francis de;Lima, Marlúcia Pereira de;Concei??o, Miri? Barbosa da;Fernandes, Hugo;
Revista Latino-Americana de Enfermagem , 2007, DOI: 10.1590/S0104-11692007000300013
Abstract: the present study aimed to describe the alcohol consumption of a hundred workers of the garbage collection service in the peripheral south region of s?o paulo, sp, brazil. the transversal study was adopted as the theoretical-methodological referential. data were collected through the questionnaire qrcap, based on the questionnaire audit. the results showed that 94% of the interviewees ingest some kind of alcoholic beverage, while 15% were considered dependents. peer pressure was pointed as the main reason to initiate the habit (46%). a total of 67% of the interviewees had already felt some symptom as the result of the ingestion of alcohol and 80% reported to be afraid of getting mentally ill due to the vice. external and internal situations were reported as the precursors of alcohol consumption. however, it was evidenced that the lack of orientation and information contributes for the prevalence of this illness.
O significado dos cuidados paliativos para os pais de crian a com cancer
Alessandra dos Santos Mabuchi,Daniel Francis de Oliveira,Marlúcia Pereira de Lima,Miri? Barbosa da Concei??o
Saúde Coletiva , 2010,
Abstract: El objetivo de esta investigación fue saber el significado de los cuidados paliativos para los padres del ni os con cáncer. Fue utilizada la Teoria Fundamentada en Dados. La colecta de datos fue hecha en un hospital de cáncer pediátrico de la ciudad de S o Paulo, Brasil, con entrevistas con doce padres de ni os con cáncer en cuidados paliativos. La investigación evidenció que los padres viven entre el miedo de la muerte y la esperanza de la curación, en un extremismo de sensaciones, donde los cuidados paliativos se consideran como esenciales para la calidad de vida de los pacientes y su familia, agregando comodidad, dignidad y relevación del sufrimiento. También fueron detectadas las limitaciones, las necesidades y las sugerencias referentes a los cuidados paliativos, qué hace posible la puesta en práctica y las estrategias que ajustan y mejoran la calidad de los cuidados ofrecidos en esta situación.
Effects of aging methods on microleakage of an adhesive system used as a sealant on contaminated surfaces
Bauer, José Roberto de Oliveira;Reis, Alessandra;Loguercio, Alessandro Dourado;Barroso, Lucia Pereira;Grande, Rosa Helena Miranda;
Journal of Applied Oral Science , 2005, DOI: 10.1590/S1678-77572005000400012
Abstract: this study evaluated the effects of aging methods on the microleakage of an adhesive system used for sealing pit and fissure surfaces. after acid etching, the occlusal surfaces of 60 permanent teeth were contaminated with 1ml of human plasma and then sealed with optibond dual cure, activated in the ramp mode (optilux 501). the sample was then divided into eight experimental and a control groups. aging was performed for 20 days in 5, 37, 55 and 5-55oc (200 cycles/day) under water contact or not. after subjecting the specimens to the agno3 technique, they were sectioned twice. dye penetration was measured and the mean values were subjected to anova. no group provided hermetic seal against dye penetration, even the control group (without treatment). significant aging x water interaction effect (p=0.033) was observed. the group without water contact at 5oc (p=0.015) showed the least dye penetration value. the results led to the main conclusions: 1) all groups showed dye penetration, even the control group; 2) storage teeth at 5oc on dry condition provided the lowest degree of dye penetration; 3) in the presence of water, the temperature variations seem not to affect the microleakage values.
Viremic blood donor found by a rapid screening method in a season of high human parvovirus B19 activity in Niterói, Rio de Janeiro, Brazil
Setúbal, Sérgio;Cárdias, Cláudia Alessandra da Silva;Oliveira, Solange Artimos de;Nascimento, Jussara Pereira do;
Memórias do Instituto Oswaldo Cruz , 2004, DOI: 10.1590/S0074-02762004000100017
Abstract: erythrovirus b19 infection is usually benign but may have serious consequences in patients with hemolytic anemia (transient aplastic crisis), immunodeficiency (in whom persistent infection can lead to chronic bone marrow failure with anemia), or who are in the first or second trimester of gestation (spontaneous abortion, hydrops fetalis, and fetal death). being non-enveloped, b19 resists most inactivation methods and can be transmitted by transfusion. b19 is difficult to cultivate and native virus is usually obtained from viremic blood. as specific antibodies may be absent, and there is no reliable immunological method for antigen detection, hybridization or polymerase chain reaction are needed for detecting viremia. a rapid method, gel hemagglutination (diamed id-parvovirus b19 antigen test), can disclose highly viremic donations, whose elimination lessens the viral burden in pooled blood products and may even render them non-infectious. in order to obtain native antigen and to determine the frequency of viremic donors, we applied this test to blood donors in a period of high viral activity in our community. positive or indeterminate results were re-tested by dot-blot hybridization. we tested 472 donors in 1998 and 831 ones in 1999. one viremic donor was found in 1999. we suggest that in periods of high community viral activity the gel hemagglutination test may be useful in avoiding highly viremic blood being added to plasma pools or directly transfused to patients under risk.
Characterization of corn landraces planted grown in the campos gerais region (Paraná, Brazil) for industrial utilization
Pinto, Alessandra Teixeira Barbosa;Pereira, Joyce;Oliveira, Tatiana Roselena de;Prestes, Rosilene Aparecida;Mattielo, Rodrigo Rodrigues;Demiate, Ivo Mottin;
Brazilian Archives of Biology and Technology , 2009, DOI: 10.1590/S1516-89132009000700003
Abstract: this work has the objective of characterizing twenty corn landraces grown in the campos gerais region (paraná state) in relation to its chemical composition (moisture, ash, protein, ether extract, dietary fiber and starch) and physical properties (weight of 1000 grains, real density, flotation index, granulometry and color). in addition, also the lab scale processing of the kernels from the varieties was carried out for producing starch; starch purity was evaluated by measuring its protein contamination. amylose contents and viscoamylograph profile were also evaluated. the results showed that the evaluated landraces have differences in chemical composition as well as in pericarp/endosperm/germ proportions and consequently it should have different industrial applications and interest for plant breeding.
ácido giberélico, bissulfureto de carbono e ácido 2-4 cloroetil fosf?nico e a dormência e produtividade de tubérculos de batata
Ayub, Ricardo Antonio;Furiatti, Rui Scaramella;Pereira, André Belmont;Reghin, Marie Y.;Banzatto, David Ariovaldo;Oliveira, Alessandra Valéria de;
Scientia Agricola , 1999, DOI: 10.1590/S0103-90161999000500001
Abstract: in order to evaluate the efficiency of giberellic acid, carbon-disulphide, and 2-4 chloro-ethyl phosphonic acid (ethrellr) on dormancy breaking and yield of potato cv. 'marijke', an essay was conducted at piraí do sul, pr, brazil, during 1990/1991. the superiority of the ethrellr treatment over giberellic acid and carbon-disulphide, and its easy application, justify its use for dormancy breaking of potato tubers, for the studied genotype and location, at the rate of 842 mg.l-1, resulting in a potato yield of 23,395 kg/ha.
Viremic blood donor found by a rapid screening method in a season of high human parvovirus B19 activity in Niterói, Rio de Janeiro, Brazil
Setúbal Sérgio,Cárdias Cláudia Alessandra da Silva,Oliveira Solange Artimos de,Nascimento Jussara Pereira do
Memórias do Instituto Oswaldo Cruz , 2004,
Abstract: Erythrovirus B19 infection is usually benign but may have serious consequences in patients with hemolytic anemia (transient aplastic crisis), immunodeficiency (in whom persistent infection can lead to chronic bone marrow failure with anemia), or who are in the first or second trimester of gestation (spontaneous abortion, hydrops fetalis, and fetal death). Being non-enveloped, B19 resists most inactivation methods and can be transmitted by transfusion. B19 is difficult to cultivate and native virus is usually obtained from viremic blood. As specific antibodies may be absent, and there is no reliable immunological method for antigen detection, hybridization or polymerase chain reaction are needed for detecting viremia. A rapid method, gel hemagglutination (Diamed ID-Parvovirus B19 Antigen Test), can disclose highly viremic donations, whose elimination lessens the viral burden in pooled blood products and may even render them non-infectious. In order to obtain native antigen and to determine the frequency of viremic donors, we applied this test to blood donors in a period of high viral activity in our community. Positive or indeterminate results were re-tested by dot-blot hybridization. We tested 472 donors in 1998 and 831 ones in 1999. One viremic donor was found in 1999. We suggest that in periods of high community viral activity the gel hemagglutination test may be useful in avoiding highly viremic blood being added to plasma pools or directly transfused to patients under risk.
Efeito do fornecimento de diferentes fontes de lipídeos na dieta sobre o consumo, a digestibilidade e o N-uréico plasmático de novilhos bubalinos em confinamento
Oliveira, Ronaldo Lopes;Assun??o, Diêni Maria Pereira;Barbosa, Marco Aurélio Alves de Freitas;Ladeira, Márcio Machado;Silva, Manoel Messias Pereira da;Mascarenhas, Alessandra Gimenez;Snel-Oliveira, Marília Viviane;Oliveira, Renata Lopes de;
Revista Brasileira de Zootecnia , 2007, DOI: 10.1590/S1516-35982007000300029
Abstract: the objective of this trial was to evaluate the effect of different dietary fat sources on intake, digestibility, and plasma urea n in feedlot water buffalo steers. thirteen castrated murrah steers averaging 297±9 kg of body weigh were assigned to one of the following three treatments: no added fat (control), soybean oil or whole soybean in a completely randomized design. the experiment lasted 84 days with three experimental periods of 28 days each. samples of feeds, orts and feces were collected in the last six days of each period for determination of intake. diets were fed twice a day (8 a.m. and 4 p.m.) as total mixed ration and the amount of feed offered was adjusted daily to yield refusals equal to approximately 10 to 20% of intake. intakes of dm and cp were greater in the control compared to treatments supplemented with fat while the opposite was observed for ether extract intake. apparent total tract digestibility of dry mater did not differ across treatments but animals fed the diet containing soybean oil had the lowest fiber digestibility. feeding whole soybean to feedlot steers had no negative impact on fiber digestibility showing that the polyunsaturated fatty acids of this fat source were less available in the rumen. it can be concluded that the higher energy content of diets supplemented with fat sources increased dm intake but feeding soybean oil reduced fiber digestibility in this study.
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