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Development of globular embryos from the hybridization between 'Pêra Rio' sweet orange and 'Ponc ' mandarin
Chagas Edvan Alves,Pasqual Moacir,Ramos José Darlan,Cardoso Pollyana
Revista Brasileira de Fruticultura , 2003,
Abstract: This research was undertaken to study the influence of different concentrations of the MT medium, sucrose, vitamins, activated charcoal and gibberellic acid (GA3) on the culture of immature embryos from the crossing between 'Pêra Rio' sweet orange and 'PONC ' mandarin. The embryos were excised under aseptic conditions and inoculated in 15 mL of the MT medium according to the following experiments: 1) MT concentrations (0%, 50%, 100%, 150% and 200%) supplemented with 0, 30, 60 and 90 g.L-1 of sucrose; 2) vitamins concentrations of the MT (0%, 50%, 100%, 150% and 200%) supplemented with 0, 30, 60 and 90 g.L-1 of sucrose; 3) activated charcoal concentrations (0, 0.5, 1, 1.5 and 2 g.L-1) supplemented with GA3 (0, 0.01, 0.1; 1 and 10 mg.L-1). After the inoculation, the embryos were kept in a growth room for 90 days at 27 ± 1oC, in a 16-hour photoperiod with 32 μmol.m-2.s-1 of irradiance. The best development of embryos at the globular stage was achieved using 50% and 100% of the MT medium plus 60 g.L-1 and 90 g.L-1 of sucrose, respectively, supplemented with 0.01 mg.L-1 of GA3. The addition of activated charcoal or vitamins in the MT medium has shown to be unnecessary to the development of globular embryos.
Composi??o do meio de cultura e condi??es ambientais para germina??o de gr?os de pólen de porta-enxertos de pereira
Chagas, Edvan Alves;Pio, Rafael;Chagas, Pollyana Cardoso;Pasqual, Moacir;Bettiol Neto, José Emílio;
Ciência Rural , 2010, DOI: 10.1590/S0103-84782010000200002
Abstract: in order to support the works with genetic improvement for pear rootstocks and adjust the pollen germination protocol for intra and inter species polinization, this study aimed to adjust the culture medium basic compounds and environmental conditions for the realization of in vitro pears pollen grains germination and viability tests of 'taiwan nashi-c' (pyrus calleryana) e 'taiwan mamenashi' (p. betulaefolia) pear rootstock. thus, five experiments were performed: 1) sucrose concentrations (0, 30, 60 and 90g l-1) and agar (4, 6, 8 and 10g l-1); 2) calcium nitrate (0, 200, 400 and 800mg l-1) and boric acid (0, 400, 800 and 1200mg l-1); 3) ph (4.0; 4.5; 5.0; 5.5; 6.0 and 6.5); 4) temperatures (20; 25; 30 and 35 ) and 5) emission time of the pollen tube (1, 2, 3, 4, 5, 6 and 12 hours after inoculation), which were assembled sequentially. the use of 10g l-1 of agar combined with 90g l-1 sucrose for the rootstock 'taiwan mamenashi' and 47.78g l-1 for the 'taiwan nashi-c', with 795 to 838mg l-1 of boric acid, under absence of calcium nitrate and ph between 5.2 and 5.8 and temperature of 28 °c, provided the best conditions to the germination of the pollen grains. the maximum percentage of germinated pollen is obtained with eight hours after inoculation for 'taiwan nashi-c' and twelve hours for 'taiwan mamenashi'.
Indolbutyric acid concentration in the propagation of mume by air layering Concentra es de ácido indolbutírico na propaga o do umezeiro por alporquia
Edvan Alves Chagas,Pollyana Cardoso Chagas,Rafael Pio,José Emílio Bettiol Neto
Semina : Ciências Agrárias , 2012,
Abstract: Some research works were developed to propagate mume by cutting, even so the results were not exciting. The objective of present work was to verify the air layering in ‘IAC-10’ mume (japanese apricot) treated with different concentration of indolbutyric acid (IBA). In woody branches of approximately one year, air layering was accomplished with a 2 cm wide ring treated with IBA (0, 1,000; 2,000; 3,000 and 4,000 mg L-1), involved with humidified sphagnum moss, covered and tied in the extremities with transparent plastic film. After 90 days, percentage of survival air layering, rooting, callous and number of roots were evaluated. According to the results, mume can be propagated by air layering with a concentration of 1000 mg L-1 of IBA in order to provide a higher number of roots and a good rooting. Alguns trabalhos foram desenvolvidos com o intuito de se propagar o umezeiro por estaquia, porém os resultados n o foram animadores. O presente trabalho teve como objetivo verificar a viabilidade da propaga o do umezeiro (Prunus mume sieb & Zucc), clone IAC-10, por alporquia. Ramos lenhosos de aproximadamente um ano de idade, localizados na parte mediana da copa, foram utilizados para a confec o dos alporques. Realizou um anel com aproximadamente dois centímetros de largura (suprimindo apenas a casca do ramo), pincelando-se com diferentes concentra es de IBA (0, 1000, 2000, 3000 e 4000 mg L-1), distribuídos em todo o anel com o auxílio de pincel. Os alporques foram envolvidos com esfagno umedecido, como substrato, cobertos e amarrados nas extremidades com plástico transparente. Após 90 dias da realiza o da alporquia, coletaram-se os seguintes dados biométricos: porcentagem de alporques vivos, enraizados, calejados e número médio de raízes por alporque. De acordo com os resultados obtidos, conclui-se que o umezeiro pode ser propagado por alporquia, devendo ser utilizado a concentra o de 1000 mg L-1 de IBA, a fim de propiciar maior enraizamento e número de raízes por alporque.
Desenvolvimento in vitro de embri?es imaturos oriundos de tangerineira 'ponc?' x laranjeira 'pera' em diferentes fotoperíodos
Pasqual, Moacir;Alves, Guilherme Pereira;Dutra, Leonardo Ferreira;Chagas, Edvan Alves;Ribeiro, Luciene de Oliveira;
Ciência e Agrotecnologia , 2003, DOI: 10.1590/S1413-70542003000300006
Abstract: it was aimed to study the influence of the photoperiod on in vitro culture of immature embryos from ‘ponc?’ mandarin and ‘pera’ sweet orange fruits. the embryos were removed from 30-40-mm wide fruits and inoculated on the ms medium, supplemented with 0.3 mg l-1 ga3 and 1 mg l-1 activated charcoal, previously sterilized at 121°c for 20 minutes. the following photoperiods were evaluated: 8, 10, 12, 14, 16, 18, and 24 hours of continuous light in 43 μm.m-2.s-1 intensity, at 27oc, in b.o.d chambers. the 16 hours continuous light was performed in growth room at 35 μm m-2 s-1. the statistical analysis used was entirely randomized, with four replications, each one constituted of three tubes. after 60 days, better results for plant height, number of leaves, above ground fresh matter weight, root length, and root fresh weight were obtained with 8, 10, 12, 14, 18 e 24 hours photoperiod. the 16 hours rendered smaller immature embryos development.
Receptividade do estigma e ajuste de protocolo para germinac?o in vitro de gr?os de pólen de citros
Darlan Ramos,José; Pasqual,Moacir; Aparecida Salles,Leila; Alves Chagas,Edvan; Pio,Rafael;
Interciencia , 2008,
Abstract: data about the viability and germination of pollen grains are essential for the success of on-field controlled hybridizing. the purpose of this work was to study the stigmas receptivity period of citrus flowers under different stages and adjust the protocol to the in vitro pollen grain germination. six essays were carried out with the aim to define the stages for collection and pollinizations, and to establish the ph and ideal sucrose, agar, calcium and boron concentrations, and temperature, for germination. for the pêra, natal and valência sweet orange varieties, manual pollinization can be performed using flowers at the "balloon" stage. for the pollen grains germination of the natal, pêra and valência varieties, the medium should be made of 100g·l-1 sucrose, 800mg·l-1 calcium, 200mg·l-1 boric acid and ph= 6.5, exception made of the pêra variety, whose germination was better at ph 5.0. a temperature of 25oc provided the best percentage of germinated pollen grains for all varieties.
Enraizamento de segmentos nodais caulinares de figueira
Pauletti, Daiana Raquel;Pio, Rafael;Barbosa, Wilson;Chagas, Edvan Alves;Kotz, Tailene Elisa;
Bragantia , 2010, DOI: 10.1590/S0006-87052010000400013
Abstract: the aim of this work was to increase the rooting of stem nodal segments of tree seedling using different concentrations of iba and injuries between the knots. stem cuttings of fig tree 'roxo de valinhos' were collected at the moment of the hibernal pruning (july), standardized with 20 cm in length, diameter of 12 mm and five knots. in half of the stakes, an injury was done between the knots [square cuts with dimensions of 1 (one) cm]. the stakes were treated with different concentrations of iba (0, 1.000, 2.000 and 3.000 mg l-1) and fully buried in the horizontal position at 5 cm of depth in moist sand under nursery (50% of brightness) condition. after 90 days, the rooting and sprouting of stakes, the length of shoots, the rooted nodes, number of roots and shoots per cutting and knot and yield of seedlings (average number of nodes rooted in sprouted cuttings) were evaluated. the rooting of nodal segments is an alternative for increasing the rooting of fig tree seedlings, being indicated an injury between knots associated to the application of 2000 mg l-1 of iba.
Enxertia em figueira 'Roxo de Valinhos' por borbulhia e garfagem
Kotz, Tailene Elisa;Pio, Rafael;Campagnolo, Marcelo Angelo;Chagas, Edvan Alves;Dalastra, Idiana Marina;
Bragantia , 2011, DOI: 10.1590/S0006-87052011000200013
Abstract: aiming to develop a protocol for grafting unrooted cuttings of 'roxo de valinhos' fig tree (ficus carica l.), two experiments were carried by applying different propagation techniques: budding and grafting. in the first experiment, cuttings of 20 cm length taken from the middle portion of the branches from june to september were grafted by bud of plate type and normal 't' type. in the second experiment, cuttings were collected in july and grafted by the cleft grafting method, by immersing cuttings in 2.000 mg l-1 iba for 10 s. control cuttings were not immersed in iba. the grafts were protected with transparent plastic bags (18 x 3 cm), for 0, 15, 30, 45 and 60 days. cuttings from both experiments were buried 2/3 of their length in sand under greenhouse conditions (50% of shading). at 60 and 120 days after grafting, percentage of buds and forks alive, buds and forks sprouted, percentage of buds and forks alive on rooted rootstocks, and sprout length were evaluated. as conclusion, grafting of 'roxo de valinhos' fig tree can be accomplished by normal "t" type budding and also by grafting, with an immersion of the cuttings in iba and graft protection for 60 days.
Tempo de estratifica??o e temperatura na porcentagem e velocidade de germina??o do marmeleiro 'Japonês'
Pio, Rafael;Abucarma, Vania Márcia;H?ring, Carla Franciele;Chagas, Edvan Alves;Barbosa, Wilson;
Bragantia , 2008, DOI: 10.1590/S0006-87052008000300027
Abstract: the quince tree has always been commercially propagated by cuttings. due to low vigor of the young plants, mainly in the first years in the orchard, a series of works was developed in brazil in order to make possible the use of the 'japonês' quince as a rootstock. the results were satisfactory but due to lack of other informations on the propagation process, the present work aimed to of verify the ideal stratification time and germination temperature on the percentage and speed of germination of 'japonês' quince tree. seeds were placed in petri dishes, between moistened cotton layers in a b.o.d. type chamber at 4 oc followed by planting in sand substrate inside plastic trays, using the same b.o.d. type chamber maintained at different temperatures (15, 20, 25 and 30 oc). counting of normal seedlings were made daily until 30 days, and at the end the speed of germination index and germination percentage were calculated. seeds of 'japonês' quince tree should be stratified for 30 days in cold temperature and sowed when the ambient temperatures are between 15 and 20 oc, to obtain maximum germination.
Enxertia intergenérica de cultivares de nespereira no porta-enxerto de marmeleiro 'japonês'
Pio, Rafael;Chagas, Edvan Alves;Barbosa, Wilson;Signorini, Guilherme;Feldberg, Nelson Pires;
Ciência e Agrotecnologia , 2010, DOI: 10.1590/S1413-70542010000600014
Abstract: in brazil, some pioneer studies were carried out using quince seedlings (cydonia oblonga mill.) as rootstock for loquat (eriobotrya japonica (thunb.) lindl.). the main advantage of this intergeneric grafting use is plant size reduction. the success of using this intergeneric grafting is related mainly to plant size reduction. therefore, the objective of this work was to study grafting techniques of loquat cultivars using 'japonese' quince tree (chaenomeles sinensis (thouin) koehne), with new option for rootstock. the one-year old 'japonese' quince seedlings (with around 110 cm of height and 0.85 cm of diameter at the grafting point, 15 cm from the soil), were kept in plastic bags (30 x 18 cm, 3 liters of capacity). the seedlings were grafted by the cleft grafting and single bud grafting methods, in two different periods: in autumn (april) and winter (july). five loquat cultivars commercially planted in brazil were used: 'mizuho', 'néctar de cristal' (iac 866-7), 'mizauto' (iac 167-4), 'mizumo' (iac 1567-411) and 'centenária' (iac 1567-420). using the single bud grafting method, there was no sprouted bud when done in autumn, and just two sprouts each of 'mizauto', 'néctar de cristal' and 'centenária' when done in winter, however, with low vigor. by the cleft grafting method, higher sprout percentage and better scions growth were observed when the seedlings were grafted in winter, especially for 'mizuho', 'centenária' and 'néctar de cristal' loquat cultivars.
Enraizamento de estacas apicais de figueira 'Roxo de Valinhos' em fun??o de época de coleta e AIB
Ohland, Tatiane;Pio, Rafael;Chagas, Edvan Alves;Barbosa, Wilson;Kotz, Tailene Elisa;Daneluz, Simone;
Ciência e Agrotecnologia , 2009, DOI: 10.1590/S1413-70542009000100010
Abstract: apical cuttings of 'roxo de valinhos' fig tree collected at different times, may present differentiated rooting, which could propitiate, in practice, the knowledge of the right moment to use the cuttings for their propagation. the aim of the present work was to verify the effect of the collection time of the cutting and the treatment with iba on the rooting of the apical cuttings of 'roxo de valinhos' fig tree. woody cuttings of apical portion of the branches were collected at the end of the first fortnight of the months may, june, july, august, and september. they were standardized at 20 cm length and 7 mm diameter. the cutting was treated or not with 2000 mg l-1 iba for 10 seconds. soon after, the cuttings were buried in a moist sand bed at nursery conditions with 50% light. after 60 days, the percentage of live cuttings and their rooting, shooting, average number of shoots and roots were evaluated. it was concluded that the apical cuttings of 'roxo de valinhos' fig tree should be collected in june and treated with 2000 mg l-1 iba.
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