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Criopreservación del nemátodo Beddingia (Deladenus) siricidicola, controlador biológico de la avispa del pino
GERDING G,MACARENA; FRANCE I,ANDRES;
Bosque (Valdivia) , 2005, DOI: 10.4067/S0717-92002005000200016
Abstract: the nematode beddingia (deladenus) siricidicola was introduced from brazil, since it is the most effective biological control agent for the wood wasp, sirex noctilio. in order to maintain its original characteristics through time, a protocol for the cryopreservation of this nematode was developed. the highest viability levels were obtained by incubating the nematodes, prior to immersion in liquid nitrogen, in 5% glycerol for several days until the suspension reached 60% glycerol. viability was assessed 24 hours later by thawing the cryotubes in 37°c tap water and immersing the opened cryotube in ringer?s solution. the mean survival after storage was 73%.
Criopreservación del nemátodo Beddingia (Deladenus) siricidicola, controlador biológico de la avispa del pino Cryopreservation of the nematode Beddingia (Deladenus) siricidicola, biological control agent of sirex wood wasp
MACARENA GERDING G,ANDRES FRANCE I
Bosque (Valdivia) , 2005,
Abstract: El nemátodo Beddingia (Deladenus) siricidicola, el más efectivo controlador biológico de la avispa del pino Sirex noctilio, fue introducido al país desde Brasil. Con el fin de mantener en el tiempo las características originales del nemátodo, se desarrolló un protocolo de criopreservación. Los mayores niveles de supervivencia fueron logrados al incubar los nemátodos en glicerol al 5% que fue concentrado hasta alcanzar el 60%, antes de almacenarlos en nitrógeno líquido. La viabilidad se evaluó 24 horas después, descongelando los criotubos por inmersión en agua a 37°C y luego depositando el criotubo abierto en solución Ringer. La supervivencia promedio después de almacenaje fue de 73%. The nematode Beddingia (Deladenus) siricidicola was introduced from Brazil, since it is the most effective biological control agent for the wood wasp, Sirex noctilio. In order to maintain its original characteristics through time, a protocol for the cryopreservation of this nematode was developed. The highest viability levels were obtained by incubating the nematodes, prior to immersion in liquid nitrogen, in 5% glycerol for several days until the suspension reached 60% glycerol. Viability was assessed 24 hours later by thawing the cryotubes in 37°C tap water and immersing the opened cryotube in Ringer’s solution. The mean survival after storage was 73%.
EVALUACION DE CEPAS NATIVAS DE Metarhizium anisopliae var. anisopliae SOBRE Otiorhynchus sulcatus Fab. (COLEOPTERA: CURCULIONIDAE)
Gerding G.,Macarena; France I.,Andrés; Cisternas A.,Ernesto;
Agricultura Técnica , 2000, DOI: 10.4067/S0365-28072000000300002
Abstract: the pathogenicity of three isolates of metarhizium anisopliae var. anisopliae against the black vine weevil (otiorhynchus sulcatus) was evaluated in a laboratory test. the isolates were applied in increasing concentrations (0 to 108 conidia ml-1) to the last instar larvae. larval mortality was evaluated for 20 days, and lethal concentration lc50 lc90, and mortality rates were calculated. the three isolates varied (p£ 0.01) in the rate of mortality at different concentrations. the lc50 and lc90 for the most virulent isolate were about 106 and 108 conidia ml-1, respectively. future field studies will determine the potential of this fungus as a biological control agent for otiorhynchus sulcatus in chile.
EVALUACION DE CEPAS NATIVAS DE Metarhizium anisopliae var. anisopliae SOBRE Otiorhynchus sulcatus Fab. (COLEOPTERA: CURCULIONIDAE) Evaluation of Chilean strains of Metarhizium anisopliae var. anisopliae against Otiorhynchus sulcatus Fab. (Coleoptera: Curculionidae)
Macarena Gerding G.,Andrés France I.,Ernesto Cisternas A.
Agricultura Técnica , 2000,
Abstract: Se evaluó en laboratorio la patogenicidad de tres aislamientos de Metarhizium anisopliae var. anisopliae sobre el gorgojo de la frutilla (Otiorhynchus sulcatus). Los aislamientos fueron aplicados en concentraciones crecientes (0 a 10(8) conidias mL-1) sobre larvas en el último estadío de desarrollo. Se evaluó durante 20 días la mortalidad de larvas, calculándose la concentración letal CL50 y CL90 , y la tasa de mortalidad. La tasa de mortalidad a distintas concentraciones fue distinta para los tres aislamientos (P<= 0,01). La CL50 y CL90 para el aislamiento más agresivo, fue de 10(6) y 10(8) respectivamente. Futuros estudios a nivel de campo permitirán determinar el potencial de este hongo como agente de control biológico de O. sulcatus en Chile. The pathogenicity of three isolates of Metarhizium anisopliae var. anisopliae against the black vine weevil (Otiorhynchus sulcatus) was evaluated in a laboratory test. The isolates were applied in increasing concentrations (0 to 10(8) conidia mL-1) to the last instar larvae. Larval mortality was evaluated for 20 days, and lethal concentration LC50 LC90, and mortality rates were calculated. The three isolates varied (P<= 0.01) in the rate of mortality at different concentrations. The LC50 and LC90 for the most virulent isolate were about 10(6) and 10(8) conidia mL-1, respectively. Future field studies will determine the potential of this fungus as a biological control agent for Otiorhynchus sulcatus in Chile.
PATOGENICIDAD DE UNA COLECCIóN DE CEPAS NATIVAS DE Metarhizium spp. Y Beauveria spp. EN Aegorhinus superciliosus, Asynonychus cervinus Y Otiorhynchus sulcatus.
France I.,Andrés; Gerding G.,Macarena; Gerding P,Marcos; Sandoval V,Alicia;
Agricultura Técnica , 2000, DOI: 10.4067/S0365-28072000000300001
Abstract: different isolates of the entomopathogenic fungi beauveria and metarhizium, collected throughout chile, were screened for three important curculionid (weevil) pests. nine beauveria and 10 metarhizium isolates were evaluated on adults of aegorhinus superciliosus, 37 beauveria isolates on asynonychus cervinus larvae and 24 metarhizium on otiorhynchus sulcatus adults. inoculated insects were observed daily and compared using a mortality index. the most effective isolates were m430 and b306 for a. superciliosus, b 179 for a. cervinus and m151b for o. sulcatus. there were also numerous strains that caused low or very reduced insect mortality, suggesting the specificity of the different fungi for different insects. the foregoing justifies the search for new and better isolates, as well as additional laboratory and field evaluation of the better strains.
Desplazamiento de Conidias de Metarhizium anisopliae var. anisopliae en Columnas de Tres Series de Suelo Displacement of Conidia of Metarhizium anisopliae var. anisopliae in Columns of Three Soil Series
Ana María Salazar P,Macarena Gerding G,Andrés France I,Jorge Campos P
Agricultura Técnica , 2007,
Abstract: El hongo entomopatógeno Metarhizium anisopliae, aislamiento Qu-M270, es específico para el control del gusano blanco del trigo (Hylamorpha elegans Burm.), el cual se alimenta de raíces de diversos cultivos y se desplaza en el perfil del suelo hasta los 25 cm de profundidad. En este estudio se evaluó el desplazamiento vertical de conidias de Qu-M270, en suspensión acuosa, a través de columnas de suelo extraídas con tubos de PVC de 30 cm de largo y 20 cm de diámetro, en tres series de suelo de la Región del Bío-Bío (36°32’ lat. Sur; 71°55’ long. Oeste), Chile. La recuperación de conidias se realizó en medio especifico para Metarhizium anisopliae, contabilizando unidades formadoras de colonias por gramo de suelo seco. La serie Quella (Aquic Durixerets) fue más restrictiva al paso de conidias, las que se concentraron en los primeros 10 cm de profundidad, a diferencia de la serie Arenales (Dystric Xeropsamments) en que se observó su presencia bajo los 15 cm y en los líquidos percolados. La serie Mirador (Ultic Palexeralf) presentó una distribución uniforme a lo largo de todo el perfil. The fungus Metarhizium anisopliae isolation Qu-M270 is a promising control agent for the wheat white grub (Hylamorpha elegans Burm.), a Chilean scarabeid species whose larvae feed on roots of diverse crops and move in soil up to a depth of 25 cm . In this study, the vertical movement of Qu- M270 conidias suspended in water was evaluated in three soil series from the Bío-Bío Region (36°32’ S lat; 71°55’ W long), Chile. Soil was obtained using PVC pipes 30 cm long by 20 cm in diameter. Conidia recovery was performed in a specific medium for Metarhizium anisopliae, by counting colony forming units in 1 g of dry soil. The Quella series (Aquic Durixerets) was more restrictive to the percolation of conidia, which were concentrated at the first 10 cm . With the Arenales series (Dystric Xeropsamments), conidia were found below 15 cm and in percolated liquids. On the other hand, the Mirador series (Ultic Palexeralf) had a uniform distribution of conidia at every depth
PATOGENICIDAD DE UNA COLECCIóN DE CEPAS NATIVAS DE Metarhizium spp. Y Beauveria spp. EN Aegorhinus superciliosus, Asynonychus cervinus Y Otiorhynchus sulcatus. Pathogenicity of a Chilean wild collection of Metarhizium spp. and Beauveria spp. on Aegorhinus superciliosus, Asynonychus cervinus and Otiorhynchus sulcatus.
Andrés France I.,Macarena Gerding G.,Marcos Gerding P,Alicia Sandoval V
Agricultura Técnica , 2000,
Abstract: Se realizó una evaluación con diferentes aislamientos de los hongos entomopatógenos Beauveria y Metarhizium, colectados a lo largo de Chile, en tres especies de curculiónidos de importancia agrícola. Nueve cepas de Beauveria y 10 de Metarhizium fueron evaluados en adultos de Aegorhinus superciliosus, 37 aislamientos de Beauveria con larvas de Asynonychus cervinus y 24 cepas de Metarhizium en adultos de Otiorhynchus sulcatus. Los insectos inoculados fueron evaluados diariamente y comparados a través de índices de mortalidad. Diferentes niveles de patogenicidad fueron detectados entre las cepas, destacándose como los más efectivos los aislamientos M430 y B306 para A. superciliosus, B179 con A. cervinus y M151b para O. sulcatus. También hubo numerosas cepas que no causaron mortalidad o ésta fue muy reducida, indicando una especificidad de los distintos hongos para diferentes insectos. Lo anterior justifica la búsqueda de nuevos y mejores aislamientos, así como la evaluación en terreno de las mejores cepas. Different isolates of the entomopathogenic fungi Beauveria and Metarhizium, collected throughout Chile, were screened for three important curculionid (weevil) pests. Nine Beauveria and 10 Metarhizium isolates were evaluated on adults of Aegorhinus superciliosus, 37 Beauveria isolates on Asynonychus cervinus larvae and 24 Metarhizium on Otiorhynchus sulcatus adults. Inoculated insects were observed daily and compared using a mortality index. The most effective isolates were M430 and B306 for A. superciliosus, B 179 for A. cervinus and M151b for O. sulcatus. There were also numerous strains that caused low or very reduced insect mortality, suggesting the specificity of the different fungi for different insects. The foregoing justifies the search for new and better isolates, as well as additional laboratory and field evaluation of the better strains.
Desplazamiento de Conidias de Metarhizium anisopliae var. anisopliae en Columnas de Tres Series de Suelo
Salazar P,Ana María; Gerding G,Macarena; France I,Andrés; Campos P,Jorge; Gerding P,Marcos; Sandoval E,Marco;
Agricultura Técnica , 2007, DOI: 10.4067/S0365-28072007000300002
Abstract: the fungus metarhizium anisopliae isolation qu-m270 is a promising control agent for the wheat white grub (hylamorpha elegans burm.), a chilean scarabeid species whose larvae feed on roots of diverse crops and move in soil up to a depth of 25 cm . in this study, the vertical movement of qu- m270 conidias suspended in water was evaluated in three soil series from the bío-bío region (36°32? s lat; 71°55? w long), chile. soil was obtained using pvc pipes 30 cm long by 20 cm in diameter. conidia recovery was performed in a specific medium for metarhizium anisopliae, by counting colony forming units in 1 g of dry soil. the quella series (aquic durixerets) was more restrictive to the percolation of conidia, which were concentrated at the first 10 cm . with the arenales series (dystric xeropsamments), conidia were found below 15 cm and in percolated liquids. on the other hand, the mirador series (ultic palexeralf) had a uniform distribution of conidia at every depth
EVALUACIóN DE DOS CEPAS DEL HONGO Metarhizium anisopliae var. anisopliae (Metsh.) PARA EL CONTROL DE LARVAS DE GUSANO BLANCO Hylamorpha elegans Burm. (COLEOPTERA: SCARABAEIDAE)
Rodríguez,Marta; France,Andrés; Gerding,Macarena;
Agricultura Técnica , 2004, DOI: 10.4067/S0365-28072004000100002
Abstract: the white grub, hylamorpha elegans burm., is a serious pest on cereals and pastures in chile. in laboratory and greenhouse studies, the pathogenicity of two metarhizium anisopliae var. anisopliae (metsh.) isolates: qu-m270 and qu-m802 on h. elegans larvae was evaluated. the first evaluation was carried out by immersion of third instar larvae in increasing suspensions, 0 to 108 conidia ml-1 of each isolate. the area under an accumulated mortality curve was calculated, observing differences (p = 0.032) between the isolates, with qu-m270 being 50% superior to qu-m802. the lc50 and lc90 for qu-m270 were 104.7 and 107.5 conidia ml-1, respectively. the greenhouse evaluation was carried out in pots with pasteurized soil and 10 larvae of h. elegans, on which four suspensions (0 to 1014 conidia ha-1) were pulverized. after thirty days, there were no significant differences in the mortality between doses of 1013 and 1014 conidia ha-1 (p < 0.05), with 95 and 94% of larval mortality, respectively, but there was a difference at 1012 conidia ha-1 (p = 0.045) with 74% mortality. it is concluded that qu-m270 was more effective in the control of h. elegans larvae.
EVALUACIóN DE DOS CEPAS DEL HONGO Metarhizium anisopliae var. anisopliae (Metsh.) PARA EL CONTROL DE LARVAS DE GUSANO BLANCO Hylamorpha elegans Burm. (COLEOPTERA: SCARABAEIDAE) Evaluation of two strains of Metarhizium anisopliae var. anisopliae (Metsh.) for control of white grub Hylamorpha elegans Burm. (Coleoptera: Scarabaeidae) larvae
Marta Rodríguez,Andrés France,Macarena Gerding
Agricultura Técnica , 2004,
Abstract: El gusano blanco, Hylamorpha elegans Burm., es una plaga grave en cereales y praderas en Chile. Se evaluó en laboratorio e invernadero la patogenicidad de dos aislamientos del hongo Metarhizium anisopliae var. anisopliae: Qu-M270 y Qu-M802, sobre larvas de tercer estadio de Hylamorpha elegans Burm. La primera evaluación se realizó por inmersión de las larvas en suspensiones crecientes de 0 a 10(8) conidias mL-1 de cada aislamiento. Se calculó el área bajo la curva de la mortalidad acumulada, observándose diferencias (P = 0,032) entre aislamientos, siendo Qu-M270 superior en un 50% a Qu-M802. Las CL50 y CL90 para Qu-M270 fueron 10(4,7) y 10(7,5) conidias mL-1, respectivamente. Las evaluaciones en invernadero se realizaron en macetas con suelo pasteurizado y 10 larvas L3 de H. elegans, sobre las cuales se pulverizaron cuatro suspensiones (0 a 10(14) conidias ha-1). Luego de 30 días, no hubo diferencias significativas en la mortalidad (P < 0,05) entre dosis de 10(13) y 10(14) conidias ha-1, con 95 y 94% de mortalidad, respectivamente, pero sí respecto a 10(12) conidias ha-1 (P = 0,045), la que fue de 74%. Se concluyó que Qu-M270 fue más efectiva en el control de larvas de H. elegans. The white grub, Hylamorpha elegans Burm., is a serious pest on cereals and pastures in Chile. In laboratory and greenhouse studies, the pathogenicity of two Metarhizium anisopliae var. anisopliae (Metsh.) isolates: Qu-M270 and Qu-M802 on H. elegans larvae was evaluated. The first evaluation was carried out by immersion of third instar larvae in increasing suspensions, 0 to 10(8) conidia mL-1 of each isolate. The area under an accumulated mortality curve was calculated, observing differences (P = 0.032) between the isolates, with Qu-M270 being 50% superior to Qu-M802. The LC50 and LC90 for Qu-M270 were 10(4.7) and 10(7.5) conidia mL-1, respectively. The greenhouse evaluation was carried out in pots with pasteurized soil and 10 larvae of H. elegans, on which four suspensions (0 to 10(14) conidia ha-1) were pulverized. After thirty days, there were no significant differences in the mortality between doses of 10(13) and 10(14) conidia ha-1 (P < 0.05), with 95 and 94% of larval mortality, respectively, but there was a difference at 10(12) conidia ha-1 (P = 0.045) with 74% mortality. It is concluded that Qu-M270 was more effective in the control of H. elegans larvae.
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