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Asociación entre el Color de la Peridermis de la Papa con Características de Importancia Industrial
Andreu,Mario Alejandro; da Silva Pereira,Arione;
Agricultura Técnica , 2007, DOI: 10.4067/S0365-28072007000100009
Abstract: the skin color of the potato (solanum tuberosum l.) is a trait of easy evaluation, which could be used as a genetic marker in breeding programs, provided its association with other characteristics, mainly tuber quality traits, were confirmed. the objective of this study was to identify the degree of association between potato skin color with tuber dry matter content and chips color. clones from six biparental crosses among six commercial cultivars with yellow and red skin and six potato commercial varieties were evaluated for two years. results showed that genotypes differed for both tuber dry matter content and chip color. tests indicated no significant association of tuber skin color with tuber dry matter content and chip color. it was concluded that potato skin color has no association with tuber quality traits and should not be considered as a morphological marker for selection of superior genotypes for tuber quality traits.
Asociación entre el Color de la Peridermis de la Papa con Características de Importancia Industrial Relationship between Potato Skin Color and Characteristics of Industrial Importance
Mario Alejandro Andreu,Arione da Silva Pereira
Agricultura Técnica , 2007,
Abstract: El color de la peridermis de los tubérculos de papa (Solanum tuberosum L.) es una característica de fácil evaluación, que podría ser usada en los programas de mejoramiento como marcador morfológico, una vez comprobada su asociación con otras características, principalmente las de calidad industrial. Con el objetivo de identificar el grado de asociación entre el color de la peridermis de los tubérculos de papa con la cantidad de materia seca y con el color de los chips, durante dos a os se analizaron clones pertenecientes a seis cruzamientos biparentales entre variedades de peridermis colorada y amarilla y seis variedades comerciales. Por los resultados, los genotipos se diferenciaban en los promedios de las dos características mencionadas, comprobándose variabilidad en las mismas. Pruebas adicionales indicaron la ausencia de una asociación significativa entre el color de la peridermis de los tubérculos de papa con los contenidos de materia seca y el color de los chips. Se concluye, por tanto, que el color de la peridermis de los tubérculos de papa no está asociada a la calidad para el procesamiento industrial y, por consiguiente, no puede ser considerada como un marcador morfológico en la selección de genotipos superiores relacionados a estas características. The skin color of the potato (Solanum tuberosum L.) is a trait of easy evaluation, which could be used as a genetic marker in breeding programs, provided its association with other characteristics, mainly tuber quality traits, were confirmed. The objective of this study was to identify the degree of association between potato skin color with tuber dry matter content and chips color. Clones from six biparental crosses among six commercial cultivars with yellow and red skin and six potato commercial varieties were evaluated for two years. Results showed that genotypes differed for both tuber dry matter content and chip color. Tests indicated no significant association of tuber skin color with tuber dry matter content and chip color. It was concluded that potato skin color has no association with tuber quality traits and should not be considered as a morphological marker for selection of superior genotypes for tuber quality traits.
The analyst's desire in the clinic of anorexia
Silva, Mariana Benatto Pereira da;Pereira, Mario Eduardo Costa;Celeri, Eloísa Helena Valler;
Revista Latinoamericana de Psicopatologia Fundamental , 2010, DOI: 10.1590/S1415-47142010000200004
Abstract: the present work deals with the issue of the analyst's desire in the psychoanalytical treatment of anorexia. it analyzes important elements to establish transference in these cases, as the pursuit of death and the choice of refusing food as a way of controlling the demands of the other. it then discusses the "analist's desire" function in this clinic. rejecting the definition of a treatment model and the structural categorization of anorexia, we can find in the cases of the girl of angouleme (charcot) and sidonie (m. mannoni) present possible subjective ways to get out of this psychopathological impasse, by means of this function.
Correlation between parameters of electrophysiological, histomorphometric and sciatic functional index evaluations after rat sciatic nerve repair
Martins, Roberto Sergio;Siqueira, Mario Gilberto;Silva, Ciro Ferreira da;Plese, José Píndaro Pereira;
Arquivos de Neuro-Psiquiatria , 2006, DOI: 10.1590/S0004-282X2006000500010
Abstract: the rat sciatic nerve is a well-established model for the study of recovery from peripheral nerve injuries. traditional methods of assessing nerve regeneration after nerve injury and repair, such as electrophysiology and histomorphometry, despite widely used in neural regeneration experiments, do not necessarily correlate with return of motor and sensory functions. the aim of this experimental study is to investigate the possible correlation between several parameters of peripheral nerve regeneration after repair of sectioned sciatic nerve in wistar rat. a two-stage approach was used to obtain 17 parameters after electrophysiological, morphometric and sciatic functional index evaluations. pearson's correlation analysis was performed between these results. only two positives correlations between different classes of peripheral nerve assessments were noted, between sciatic functional index and proximal nerve fiber diameter (r=0.56, p<0.01) and between sciatic functional index and distal fiber diameter (r=0.50, p<0.01). the data presented in our study demonstrates that there is a poor correlation between the sciatic functional index and outcome measures of electrophysiological and morphometric evaluations.
Production and characterization of Fusarium verticillioides exoantigens/ Produ o e caracteriza o de exoantígenos de Fusarium verticillioides
Paula Garcia Meirelles,Luciana Biazon,Mario Augusto Ono,Adriana Pereira da Silva
Semina : Ciências Agrárias , 2006,
Abstract: Fusarium verticillioides Sacc. Niremberg (=F. moniliforme Sheldon) is a primary corn pathogen and the main fumonisin producer. This fungus can cause significant economical losses for the farmers, grain processors, animal producers and risk for human and animal health. Several methods for mould detection have been used, however most of these are time-consuming and require trained staff. Otherwise, immunoassays (particularly enzyme-linked immunosorbent assay, or ELISA) provide several advantages and potential for use in rapid quality control. In this work exoantigens from eight F. verticillioides isolates were obtained for further production of polyclonal antibodies. The electrophoretic profile of these antigens showed protein bands with molecular mass ranging from 17 to 170 kDa. The antigens from 3 isolates (97K, 113F and 162A) were selected for polyclonal antibodies production, based on protein concentration and number of bands. Antiserum against F. verticillioides 97K exoantigens, which showed the highest titre in indirect ELISA (1:12.800), has potential for the immunodetection of F. verticillioides. Fusarium verticillioides Sacc. Niremberg (=F. moniliforme Sheldon) é um patógeno primário de milho e principal produtor de fumonisinas. Este fungo pode causar perdas econ micas significativas para produtores e processadores de gr os, criadores de animais, além de representar sérios riscos à saúde humana e animal. Diversos métodos para a detec o de fungos têm sido utilizados, porém a maioria demanda tempo e pessoal treinado. Por outro lado, os métodos imunológicos, particularmente os ensaios imunoenzimáticos, apresentam diversas vantagens para o emprego rápido em controle de qualidade. Neste trabalho, foram obtidos exoantígenos de 8 isolados de F. verticillioides para a produ o de anticorpos policlonais. O perfil eletroforético dos antígenos apresentou bandas com massas moleculares aparentes variando entre 17 e 170 kDa. Os antígenos de 3 isolados (97K, 113F e 162A), tendo como base a concentra o de proteínas e número de bandas, foram escolhidos para a produ o de anticorpos policlonais. O soro imune anti-97K, com maior título no ELISA indireto (1:12.800), apresenta potencial para a imunodetec o de Fusarium verticillioides.
Express o de caracteres de tubérculos em fun o do tamanho de recipiente usado no cultivo de batata na gera o de plantulas Expression of tuber traits as a function of pot size used to grow potato seedlings
Mario Alvaro Aloisio Verissimo,Arione da Silva Pereira,Sergio Delmar dos Anjos e Silva,Laerte Reis Terres
Revista Ceres , 2012,
Abstract: No melhoramento genético de batata, para alguns caracteres, é possível eliminar genótipos inferiores já nas gera es iniciais, o que permite reduzir o número de genótipos avaliados a campo, proporcionando redu o de custos. O objetivo deste trabalho foi verificar a influência do tamanho de vaso na express o de caracteres de tubérculo, em famílias de batata, na gera o de plantula. Em casa de vegeta o, dez famílias de batata foram avaliadas em dois tamanhos de recipientes, vaso grande (1 L) e vaso pequeno (250 ml). Os caracteres de tubérculos avaliados foram: cor, aspereza, profundidade de olho, sobrancelha, formato, uniformidade de formato, apontamento, curvatura, achatamento, aparência geral, massa por planta, massa média e número de tubérculos. O vaso grande proporciona maior express o da varia o genética para os caracteres profundidade de olho, sobrancelha, curvatura, achatamento, aparência geral e todos os componentes de rendimento. As maiores médias de produ o de tubérculos em número, tamanho e massa foram obtidas no vaso grande, enquanto o maior coeficiente de varia o genético para os caracteres formato, uniformidade de formato e apontamento de tubérculo foi obtido no vaso pequeno. In potato breeding, the exclusion of inferior genotypes by selection in early generations provides more versatility and cost reduction. The objective of this study was to evaluate the influence of pot size on the expression of tuber traits in potato families grown as seedling generation. Ten potato families were planted in two pot sizes, large (1 L) and small (250 ml), in the greenhouse. The following tuber traits were evaluated: color, roughness, depth of eye, eyebrow, shape, uniformity of shape, pointing, curvature, flattening, tuber general appearance, total mass per plant, average tuber weight and number of tubers. The larger pot provided greater expression of genetic variation for the depth of eye, eyebrow, curvature, flattening, and tuber general appearance of all yield components. Higher means for tuber number, tuber size and tuber mass were obtained in larger pots, while higher genetic coefficient of variation for tuber shape, uniformity of shape and tuber pointing were observed in the small pots.
Express?o de caracteres de tubérculos em fun??o do tamanho de recipiente usado no cultivo de batata na gera??o de plantulas
Verissimo, Mario Alvaro Aloisio;Pereira, Arione da Silva;Silva, Sergio Delmar dos Anjos e;Terres, Laerte Reis;Ney, Vicenti Gon?alves;Silva, Giovani Olegario da;
Revista Ceres , 2012, DOI: 10.1590/S0034-737X2012000600008
Abstract: in potato breeding, the exclusion of inferior genotypes by selection in early generations provides more versatility and cost reduction. the objective of this study was to evaluate the influence of pot size on the expression of tuber traits in potato families grown as seedling generation. ten potato families were planted in two pot sizes, large (1 l) and small (250 ml), in the greenhouse. the following tuber traits were evaluated: color, roughness, depth of eye, eyebrow, shape, uniformity of shape, pointing, curvature, flattening, tuber general appearance, total mass per plant, average tuber weight and number of tubers. the larger pot provided greater expression of genetic variation for the depth of eye, eyebrow, curvature, flattening, and tuber general appearance of all yield components. higher means for tuber number, tuber size and tuber mass were obtained in larger pots, while higher genetic coefficient of variation for tuber shape, uniformity of shape and tuber pointing were observed in the small pots.
Análise quantitativa da camada de fibras nervosas da retina de c?es normais e glaucomatosos através da polarimetria a laser
Martins, Ana Lucia Braga;Garcia, Gustavo Adolfo;Pereira, Jorge da Silva;Rodriguez, Sergio;Rivera, Alejandro;Vianna, Luiz Felipe Castro Graeff;
Brazilian Journal of Veterinary Research and Animal Science , 2003, DOI: 10.1590/S1413-95962003000600003
Abstract: once the glaucomatous damage is progressive and irreversible, studies on the glaucoma onset as well as its development have been discussed. it is known that the early diagnostisis is extremely important to the stabilization and treatment. the retinal nerve fibers layer thickness analysis ''in vivo'' was proposed in human ophthalmology in order to establish the thickness changing, due to glaucoma, and have shown that such findings can be even detected six years priors to the first clinical signs. however, in veterinary medicine, such data need to be investigated and discussed. this study used two groups of dogs, a glaucomatous group and a normal group, that had been submitted to the retinal nerve fibers layer analysis through the gdx nerve fiber analyzer. statistical data showed that the nerve fiber layer of the glaucomatous group was thinner (p < 0.05), sustaining ganglion cells axons loss in glaucomatous eyes, compared to normal eyes.
Electrophysiologic assessment of regeneration in rat sciatic nerve repair using suture, fibrin glue or a combination of both techniques
Martins, Roberto Sergio;Siqueira, Mario Gilberto;Silva, Ciro Ferreira da;Godoy, Benedito Ortiz de;Plese, José Píndaro Pereira;
Arquivos de Neuro-Psiquiatria , 2005, DOI: 10.1590/S0004-282X2005000400009
Abstract: we evaluated the repair of seccioned rat sciatic nerve by the comparison of electrophysiologic parameters. the repair was effected with suture (group a), fibrin glue (group b) or a combination of both techniques (group c). the amplitude, latency and conduction velocity of the motor and nerve action potentials were assessed before the nerve section and at reoperation after 24 weeks. there was no difference between the groups when the nerve action potential was evaluated. rats of group b presented better results than those of group a (p<0.05) when latency and the nerve conduction velocity assessed at the reoperation, and the ratio between the conduction velocity at the reoperation and before the nerve section in the motor action potential evaluation were measured. animals of group c presented better results than those of group a when the ratio between the conduction velocity of motor action potential at the reoperation and before the nerve division was considered (p<0.05). no difference between groups b and c was found. we conclude that repair using fibrin glue presented better results than suture following transection of sciatic nerve when the motor action potential was evaluated in the rat experimental model.
Resultados das técnicas de reprodu??o assistida em mulheres doadoras de oócitos no ciclo de tratamento
Cavalcante, Edvaldo;Juliano, Yara;Pereira, Dirceu Mendes;Catafesta, Edir;Shimabukuro, Litsuko;Cury, Maria Cristina Faria da Silva;Cavagna, Mario;
Revista Brasileira de Ginecologia e Obstetrícia , 2005, DOI: 10.1590/S0100-72032005001100005
Abstract: purpose: to compare the outcome of treatment in patients undergoing assisted reproductive technology (art) cycles who donated eggs during their own art treatment with the outcome of patients undergoing art without egg donation. methods: we studied retrospectively the pregnancy and implantation rates of 50 patients who donated eggs during the course of their art treatment (donor group), and the pregnancy and implantation rates of 50 patients who underwent art cycles and kept all their eggs (non-donor group). between the years 2001-2003. the inclusion criteria used were as follows: age <35 years old, normal menstrual cycles, fsh<10 miu/ml, first attempt of art treatment and more than six mature oocytes retrieved. the results were analyzed statistically using the c2 test. results: both groups were comparable in terms of age, indication, and duration of infertility. the mean age was 30.6 years for the donor group and 31.1 years for the non-donor group. all the patients of the donor group produced more than 6 eggs. from the donor group we collected 590 oocytes; 215 eggs were donated to recipients (36.5%) and 152 embryos were transferred. a total of 15 pregnancies were achieved (pregnancy rate per transfer: 30%); the implantation rate was 11.2% and there were 2 miscarriages (miscarriage rate: 13.3%). from the non-donor group, 545 oocytes were collected and 153 embryos were transferred. a total 17 pregnancies were achieved (pregnancy rate per transfer: 34%); the implantation rate was 14.3% and there were 3 miscarriages (miscarriage rate: 17.6%). the pregnancy and implantation rates were similar in both groups, and there were no significant statistical differences regarding the miscarriage rate (p>0.05). conclusion: this study suggests that in patients who produce more than 6 oocytes, egg donation in the treatment cycle does not influence adversely the outcome of art cycles and does not increase the miscarriage rate.
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